Thermophilus muts
Webb10 jan. 2007 · T.thermophilus is a Gram-negative eubacterium that can grow at temperatures greater than 75°C ( 17 ). In general, proteins isolated from T.thermophilus … All living organisms have DNA repair systems to counteract DNA damage caused by sunlight, chemical agents and DNA replication errors (1). DNA repair … Visa mer
Thermophilus muts
Did you know?
Webb1 apr. 2003 · Thermus thermophilus MutS protein hydrolyzed ATP to ADP and Pi, and its activity was maximal at 80°C. The kinetic parameters of the ATPase activity at 65°C were Km = 130 µM and kcat = 0.11 s−1. Webb1 mars 2011 · MutS family proteins are widely distributed in almost all organisms from bacteria to human and play central roles in various DNA transactions such as DNA …
Webb21 mars 2013 · Effects of MutS on gene amplification from genomic DNA. (A) A 423-bp region of ttha1806 of T. thermophilus was amplified in the presence of TthMutS. The 500-bp DNA ladder is indicated by the lane marked “M”. A second aliquot of TthMutS was added to the reaction at the end of the WebbThermus thermophilus MutS, a thermostable mismatch-recognizing protein, is utilized in PCR to suppress nonspecific amplification by preventing synthesis from mismatched …
Webb25 maj 2001 · T. thermophilus HB8 MutS protein was purified as described (Takamatsu et al., 1996). A mutant protein K597A, where the lysine residue at position 597 is replaced by alanine, was constructed using the polymerase chain reaction. DNA manipulations were performed using standard procedures (Sambrook et al., 1989). WebbThe mutS gene from the thermophilic bacterium Thermus thermophilus was PCR amplified, cloned, and expressed in Escherichia coli. The recombinant MutS protein containing an oligohistidine domain at the N-terminus was purified in a single step by Ni(2+) affinity chromatography to apparent homogeneity.
Webb10 sep. 2009 · We previously reported the construction of a plasmid vector carrying mutS-lacZ, a fusion gene designed to obtain a bifunctional chimeric protein consisting of MutS (mismatch binding protein) and β-galactosidase (Sachadyn et al., 2007).The fusion gene was created by in-frame cloning of E. coli lacZ gene into an expression plasmid carrying …
WebbThermus thermophilus is a Gram-negative bacterium used in a range of biotechnological applications, including as a model organism for genetic manipulation, structural … other than these meaningWebb21 juli 2013 · Thermus thermophilus MutS, a thermostable mismatch-recognizing protein, is utilized in PCR to suppress nonspecific amplification by preventing synthesis from mismatched primers. T. thermophilus RecA also decreases nonspecific amplification by promoting proper hybridization between the primer and template. other than thingiverseWebbuse of MutS proteins from hyperthermophiles, including Thermus thermophilus MutS (TthMutS), to reduce the misincorporation of oligonucleotides and remove mismatched oligonucleotides from DNA mixtures during PCR. Since then, several studies have reported some success in removing mismatched oligonucleotides from DNA mixtures with MutS … rocking horse high chair desk plansWebb15 sep. 1998 · MutS protein binds to DNA and specifically recognizes mismatched or small looped out heteroduplex DNA. In order to elucidate its structure-function relationships, the domain structure of Thermus thermophilus MutS protein was studied by performing denaturation experiments and limited proteolysis. rocking horse home depotWebb1 mars 1996 · Here, we describe the isolation and characterization of a thermostable MutS homolog from Thermus aquaticus YT-1. Sequencing of the gene predicts an 89.3-kDa … rocking horse high chair patternsWebb15 sep. 1998 · MutS protein binds to DNA and specifically recognizes mismatched or small looped out heteroduplex DNA. In order to elucidate its structure-function relationships, … other than those mentioned aboveWebb28 apr. 1997 · MutS as a mismatch binding protein is a promising tool for SNP detection. Green fluorescent protein (GFP) is known as an excellent reporter domain. We … other than to do