WebRecommended For. Applications requiring a premium-quality, multi-purpose hose with … WebA transcription terminator – usually just called a terminator – is a DNA sequence that …
Plant terminators: the unsung heroes of gene expression
WebThe key role of terminators on the expression and post-transcriptional gene silencing of … Transformation plasmids were introduced into Agrobacterium tumefaciens C58 quimiocompetent cells and plated in LB medium supplemented with Rifampicin (25 µg/ml) (Sigma-Aldrich) and Kanamycin (50 µg/ml). A single transformant colony was grown in 200 mL LB medium supplemented with the same … See more GoldenBraid2.0 (GB) cloning system was used for cloning. Plasmids GB0030, GB0036, GB0035, GB0235 and GB0466 were used for … See more Arabidopsis thaliana Columbia-0 accession (Col-0) seedlings were grown in plates in MS medium with 1% sucrose in Corning®square … See more DNA from 10 day-old seedlings and leaves of 28 day-old plants was extracted using a DNeasy Plant Mini Kit (Qiagen). Bisulfite treatment was done … See more For luciferase imaging, 30 seedlings per line were sowed in plates while 5 leaves from different plants were put on a plate to analyze LUC activity. d-Luciferin Firefly, potassium salt … See more foray medium pt
Improvement of the transient expression system for …
WebNov 1, 2024 · In plants, GUS, LUC, and GFP are extensively used as the reporters or selectable markers. Technically, GUS, with the presence of glucuronides, and LUC, with the presence of luciferin, can be... WebThe role of terminators is more commonly associated with the polyadenylation and 3′ end formation of new transcripts. Recent evidence, however, suggests that this regulatory region can have a dramatic impact … WebMar 22, 2024 · The 520402000 drop terminator you will need to contact [email protected] for pricing and availability. Published for: Schneider Electric Sverige Publicerad den: 3/22/2024 Senast ändrad den: 3/22/2024 elite fitness wairau