Biotin-coupled mirna capture
WebDec 12, 2024 · Streptavidin coupled to a solid matrix of beads allows the specific capture of biotinylated RNA easily. To demonstrate the suitability of biotinylated cap analogues for the study of protein–RNA interactions we established a combined protocol including ultraviolet (UV) cross-linking, and affinity purification of biotinylated-capped RNA. WebFeb 12, 2024 · Biotin-coupled miRNA capture assay. The biotin-labeled circHIPK3 and control probes were synthesized by GenePharma (Shanghai, China). In brief, biotin-labeled circHIPK3 and control probes were …
Biotin-coupled mirna capture
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WebNov 23, 2024 · Biotin-coupled miRNA capture, fluorescence in situ hybridization and luciferase reporter assays were conducted to confirm the relationship between circMMP9 and miR-124. In this study, we screened differentially expressed circRNAs and identified circMMP9 in GBM. We found that circMMP9 acted as an oncogene, was upregulated in … WebJun 19, 2024 · Biotin-coupled miRNA capture. The biotin-coupled miRNA pull-down assay was performed as previously described 11. Briefly, 3′-end biotinylated miR-587 mimics (Ribio, Guangzhou, China) were ...
WebMay 31, 2024 · The biotin-coupled miRNA capture assay was performed as previously described. 17 In brief, miR-206 mimics and miRNA negative control were labeled with biotin at the 3′ end and transiently transfected into LoVo and HCT116 cells. After 24 hrs of culture, the cells were harvested and lysed in a lysis buffer, followed by incubation with ... WebMay 26, 2024 · We conducted biotin-coupled miRNA capture and FISH to confirm the sponge effect of Cdr1as. Biotin-coupled miR-1270 analogously captured more Cdr1as than biotin-coupled NC. This phenomenon indicates that miR-1270 could bind to Cdr1as (Fig. 3G). PCR experiments were conducted to find out the relative abundance of miR-1270 in …
WebNov 17, 2024 · In addition, a biotin-coupled miRNA capture assay was performed using a biotin-coupled miR-185-3p probe, followed by qRT-PCR. It was observed a fourfold enrichment of circ_0008494 in the miR … WebMiR-4306 response elements were predicted by bioinformatics algorithm and identified using dual-luciferase reporter, RNA immunoprecipitation, and biotin-coupled miRNA capture assays. Results CircPDE4D was markedly downregulated in OA cartilages and interleukin (IL)-1β-stressed human normal chondrocytes (HNC).
WebJan 1, 2016 · Therefore, to identify all miR-96 targets (the miR-96 targetome) in an unbiased manner, we undertook a biotin-miRNA (bi-miR) capture and streptavidin pulldown …
WebJan 16, 2024 · RNA immunoprecipitation (RIP) and biotin-coupled miRNA capture. g for 20 min. Western blot analysis. The total protein of 95D cells were exacted using protein extraction reagent (Thermo Scientific ... how many pixels can a human seeWebNov 9, 2024 · On the contrary, some studies argued that biotin moiety when attached to the 3′ end of the miRNA impede its association with AGO2. 41 Hence, biotin-labelled miRNA was not co-IPed with AGO2. 28 The negative result could be due to low transfection efficiency or post-translational modification of AGO2 that can hamper miRNA-AGO2 … how clean is the human mouthWebOct 23, 2024 · Biotin-coupled miRNA capture. The biotin-coupled miRNA pull-down assay was performed as described previously by … how many pixels does 1080p haveWebAug 17, 2024 · To validate the interactions between circCDYL and its targets, we performed RNA fluorescent in situ hybridization, biotin-coupled miRNA capture assay and biotin-coupled probe pull-down assay. Tight … how clean is snowWebDec 18, 2024 · Biotin-coupled miRNA capture. The 3′ end biotinylated miRNA mimics or control RNA (Ribio, Guangzhou, China) were transfected into ccRCC cells for 48 h before harvest. Then biotin-coupled RNA complex was pulled down by incubating the cell lysates with streptavidin-coated magnetic beads (Life Technologies). The abundance of … how clean is switzerlandWebJun 25, 2024 · Biotin-coupled miRNA capture. The biotin-coupled miRNA pull-down assay was performed using previously reported method 8. Briefly, the 3′-end biotinylated … how many pixels can human eyes seeWebJun 19, 2024 · Biotin-coupled miRNA capture. The biotin-coupled miRNA pull-down assay was performed as previously described 11. Briefly, 3′-end biotinylated miR-587 mimics (Ribio, Guangzhou, China) were ... how many pixels are there in one megapixel